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baf219 anti p40 biotinylated pab  (R&D Systems)


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    Structured Review

    R&D Systems baf219 anti p40 biotinylated pab
    Baf219 Anti P40 Biotinylated Pab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+il+12/Human+IL-12+Biotinylated+Antibody/us12559552-924-5-9
    Average 93 stars, based on 26 article reviews
    baf219 anti p40 biotinylated pab - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Activation of microglia and astrocytes by CpG oligodeoxynucleotides.
    Article Snippet: Bacterial DNA and synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs stimulate cells of the immune system to secrete a variety of cytokines and chemokines.. This function can be carried out by microglia and astrocytes in the CNS.. To evaluate the effect of CpG ODN on microglia and astrocytes, puri®ed cells were isolated and cultured in vitro.

    Staining:

    Article Title: Characterization of the Myeloid Cell Populations’ Resident in the Porcine Palatine Tonsil
    Article Snippet: Following 4 h of culture, GolgiPlug ® (BD Biosciences) was added and cells were incubated for a further 6 h. Cells were then stained as described above with the exception of applying non-biotinylated anti-Syn-CAM (TSLC1/CADM1) (Clone 3E1; MBL) (Caltag Medsystem) labeled with anti-chicken IgY APC (Jackson ImmunoResearch, Newmarket, UK) and CD163 conjugated with Zenon anti-mouse IgG1 APC AlexaFluor750 (Life Technologies, Paisley, UK). .. For intracellular staining, cells were treated with BD Cytofix/CytopermTM (BD Biosciences, Oxford, UK) for 20 min at 4°C washed with BD Perm/WashTM before staining with either biotinylated anti-IL-12 (R&D Systems, Abingdon, UK) or directly conjugated anti-TNF-α Brilliant Violet 605 (eBioscience, Hatfield, UK) in Perm/WashTM buffer. ..

    Article Title: Characterization of the Myeloid Cell Populations' Resident in the Porcine Palatine Tonsil.
    Article Snippet: Following 4 h of culture, GolgiPlug® (BD Biosciences) was added and cells were incubated for a further 6 h. Cells were then stained as described above with the exception of applying nonbiotinylated anti-Syn-CAM (TSLC1/CADM1) (Clone 3E1; MBL) (Caltag Medsystem) labeled with anti-chicken IgY APC (Jackson ImmunoResearch, Newmarket, UK) and CD163 conjugated with Zenon anti-mouse IgG1 APC AlexaFluor750 (Life Technologies, Paisley, UK). .. For intracellular staining, cells were treated with BD Cytofix/CytopermTM (BD Biosciences, Oxford, UK) for 20 min at 4°C washed with BD Perm/WashTM before staining with either biotinylated anti-IL-12 (R&D Systems, Abingdon, UK) or directly conjugated anti-TNF-α Brilliant Violet 605 (eBioscience, Hatfield, UK) in Perm/WashTM buffer. ..



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